利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒   总被引：1，自引：4，他引：1  

吕宏旭  汪岷  李红岩  郭华荣  张士璀  包振民  周丽 《中国海洋大学学报(自然科学版)》2003,33(2):233-239

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。  相似文献   

黑鲷早期鳞被的形成     

丁彦文  李加儿  区又君 《热带海洋学报》1991,(3)

黑鲷Sparus macrocephalus(Basilewsky)幼苗鳞被最早出现的部位是在躯干部胸鳍基后至肛门直上方的侧线处。黑鲷只具一个鳞被起始中心。最早出现鳞片的个体为体长9.5—14.1mm,平均11.51±0.85mm,全长11.2—16.5mm,平均13.65±1.01mm,孵后日龄23—35d。鳞被发育完全时的最小个体为体长12.1—18.5mm,平均14.57±1.55mm,全长14.8—23.5mm,平均17.99±2.17mm,孵后日龄27—42d。  相似文献   

Variation in top-down and bottom-up control of marine bivalves at differing spatial scales   总被引：3，自引：0，他引：3  

Seitz  R. D.; Lipcius  R. N. 《ICES Journal of Marine Science》2001,58(3):689-699

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Jellyfish patch formation investigated by aerial photography and drifter experiment     

Shinya Magome  Tomohiro Yamashita  Takeshi Kohama  Atsushi Kaneda  Yuichi Hayami  Satoru Takahashi  Hidetaka Takeoka 《Journal of Oceanography》2007,63(5):761-773

Jellyfish patch formation is investigated by conducting a drifter experiment combined with aerial photography of a sustained patch of the moon jellyfish in Hokezu Bay, Japan. Jellyfish patches are aggregations of individuals that are caused by a combination of swimming (active influence) and advection by currents (passive influence). The drifter experiment involved the injection of 49 drifters around a distinct surface patch of jellyfish within an area of approximately 300 m × 300 m. The drifters’ motion, caused only by the passive influence, was recorded in a series of 38 aerial photographs taken over approximately 1 h. The ambient uniform current field larger than the patch scale was estimated from the movement of the centroid position of drifters, while the distribution of horizontal divergence and relative vorticity around the patch was estimated from the time-derivative in areas of triangles formed by the drifters. The centroid positions of both drifters and patches moved stably toward the bay head at different speeds. The difference vector between the patch and drifter centroids was directed to the sun, and was opposite to the ambient current. The distributions of vorticity and divergence around patches exhibited inhomogeneity within the patch scale, and the drifters in this nonuniform current field aggregated near the convergence area within 1 h. The results suggest that horizontal patch formation is predominantly influenced by passive factors at the surface of Hokezu Bay. Furthermore, the upward swimming against downwelling may make sustained patch in surface layer.  相似文献   

Effects of organochlorine compounds on cytochrome P450 aromatase activity in an immortal sea turtle cell line     

Keller JM  McClellan-Green P 《Marine environmental research》2004,58(2-5):347-351

Many classes of environmental contaminants affect the reproductive function of animals through interactions with the endocrine system. The primary components affected by endocrine active compounds (EACs) are the steroid receptors and the enzymes responsible for steroidogenesis. This study sought to develop an in vitro model for assessing EAC effects in sea turtles by examining their ability to alter cytochrome P450 aromatase (CYP19) activity. Aromatase is the enzyme responsible for the conversion of testosterone to estradiol. This enzyme is critical in the sexual differentiation of reptiles which demonstrate temperature-dependent sex determination. An immortal testis cell line GST-TS from a green sea turtle was grown in culture at 30 degrees C in RPMI 1640 media. The cells were exposed to three known aromatase inducers; dexamethasone (Dex), 8Br-cyclic AMP, or human chronic gonadotropin (HCG) and one aromatase inhibitor 4-androstenol-dione (4-OHA). In addition, the GST-TS cells were exposed to 0.1-30 microM atrazine and 3-100 microM 4,4'-DDE. The inducing compounds that have been shown to increase aromatase activity in other systems failed to induce aromatase activity in the GST-TS cells, yet exposure to the inhibiting compound, 4-OHA, did result in a significant reduction. Atrazine (0.1, 1.0 and 10 microM) significantly induced aromatase activity following a 24 h exposure, and 4,4'-DDE inhibited the activity but only at cytotoxic concentrations (100 microM). Based on these results, this in vitro model can be useful in examining the endocrine effects of EACs in sea turtles.  相似文献   

南黄海表层沉积物中氮的区域地球化学特征   总被引：5，自引：0，他引：5  

吕晓霞  宋金明 《海洋科学进展》2003,21(2):174-180

通过分级浸取分离的方法获得了南黄海表层沉积物不同形态的氮，首次探讨其分布的区域性特征：南黄海表层沉积物中各形态氮在不同测线上均呈现出波状分布。B测线的基本上呈现出中部海域高，两边海域低，而西部海域又略高于东部海域的趋势，D和E测线的基本上呈现出西高、东低的趋势，C测线的则呈现出西低、东高的趋势，F测线的变化趋势比较平缓，从整体上看，SOEF—N和TN的分布呈现出西部海区比东部海区低的趋势。受各测线上沉积物种类差异的影响，自北向南各形态氮的变化幅度逐渐减小，其中SOEF-N在各测线上的变化幅度最大，且自北向南降幅最大。受水动力及沉积物粒度的影响，不同形态氮在黄海槽内各站位沉积物中的含量较高。沉积物中各形态氮在不同测线上的分布还表明，陆源输入对南黄海沉积物中氮测线分布有较小的影响，海洋自生氮对南黄海表层沉积物中氮的形态、含量与分布具有重要影响。  相似文献   

Multiscale Terrain Analysis of Multibeam Bathymetry Data for Habitat Mapping on the Continental Slope   总被引：2，自引：0，他引：2  

Margaret F. J. Wilson  Brian O'Connell  Colin Brown  Janine C. Guinan  Anthony J. Grehan 《Marine Geodesy》2007,30(1):3-35

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一维扩散方程整体观测资料下的初值变分同化反演     

张瑰 《海洋预报》2006,23(Z1):34-41

本文考虑一维扩散方程的反问题,利用变分同化方法通过观测资料来确定方程中的未知初值,通过分析观测误差对于初值误差的影响,证明变分同化初值收敛于原问题的真实参数,并得到了参数的收敛精度。同时将得到的初值代入预报模式中,得到预报解,并分析了预报解的收敛性和预报误差。  相似文献   

一种基于分割图斑的海岸带遥感图像分类方法     

吴均平  毛志华  陈建裕  潘德炉 《海洋学研究》2006,24(2):70-78

传统的基于象元的分类方法在处理海岸带这类动态多变、混杂度大的地物时常出现“椒盐现象”,难以矢量化形成专题图。提出了一种新的遥感图像分类方法——基于分割图斑的分类法,即选择一个合适的尺度,利用光谱信息按照一定的策略将图像分割为一系列的图斑,并确保图斑内大多数象元的光谱特征相近,分别对图斑内诸象元进行统计,求出各个波段的均值,用该均值替换图斑内所有象元的原始亮度值,这样分类时可以确保同一图斑内的所有象元被分到同一个类别中,从而有效地避免了“椒盐现象”,使分类结果便于矢量化成图,也便于对特定地物的提取。实验结果表明,该方法还能在一定程度上提高分类精度。  相似文献   

使用GAMIT进行高精度基线向量解算的方法与实践   总被引：2，自引：0，他引：2  

徐杰  任超  孟黎 《海洋测绘》2007,27(6):29-32

GAM IT是国际上常用的GPS定位定轨软件,但由于其安装的平台(UN IX/LINUX)与我们通用的W indow s平台有所差异,使得GAM IT的入门较难。因此本文结合具体实例,介绍GAM IT的安装、基本操作以及应用GAM IT进行高精度基线向量解算,供初学者参考。  相似文献